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KMID : 0374019800030030113
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Ewha Medical Journal
1980 Volume.3 No. 3 p.113 ~ p.118
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The Effects of Vitamin B Complexes on AAF Hydroxylation by Rat Liver Microsomal Cytochrome P-450
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Abstract
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The effects of vitamin B complexes on tpth ring and N-hydroxylation of 2-acetylaminofluorene by rat hepatic microsomal fraction were studied.
In the presence of thiamine-Hcl. during incubation, the total hydroxy-AAF was increased by 50.3%. 0.1mM and 1.OmM riboflavin inhibited only total hydroxy AAF with 56.7% and 85.0% whereas N-hydroxy AAF was increased ´to some extent. 1.Om-M niacin did not have much effect on the total hydroxy-AAF, but 0. ImM niacin decreased the total dydroxy-AAF by 28.6%. Presence of p-aminobenzoic acid, calcium pantothenate, pyridoxal-Hcl or vitamin B12 inhibited the total hydroxy-AAF to some extent. But the ratio of ring-and N-AAF hydroxylation was not changed by these vitamin B complexes.
Our present results suggest. that vitamin B complexes were not effective in vitro metabolism of 2-AAF to N-hydroxy AAF(activation step of AAF) by a cytochroine p-450 dependent mixed function oxidase system.
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